flowAI report

Quality control analysis

Summary

The anomalies were removed from: Flow Rate, Flow Signal and Flow Margin
Anomalies detected in total: 68.98 %
Number of high quality events: 319975

Flow rate check

17.17 % anomalies detected in the flow rate check.

The plot reconstructs the flow rate with a resolution of 1/10 of a second. Anomalies in the flow rate are identified with an algorithm based on the generalied ESD outlier detection method. The anomalies are circled in green.

Signals acquisition check

65.97 % anomalies detected in the signal aquisition check.

The more stable region selected consistent for all channels is highlighted in yellow. If the removal of outliers has been required before the execution of the changepoint analysis, the detected outliers are cicled in green.

The FCS file was divided in 2064 bins, hence each bin contains 500 event(s). The stable region is located between the bins 1 and 702.

More info on the changepoints detected

Changepoints detected in channels with shifts in signal acquisition:

##                 1    2    3
## 488 525/50-A  702 1458 1792
## 488 715/30-A  759 1564     
## 405 450/50-A 1562          
## 405 515/20-A  764 1638     
## 405 605/40-A 1513          
## 405 670/30-A  839 1565     
## 405 710/40-A 1558          
## 405 780/60-A 1423          
## 355 515/30-A 1708          
## 355 560/40-A 1450          
## 640 670/30-A  820 1598     
## 640 730/35-A  950 1687     
## 640 780/60-A  971          
## 561 585/15-A 1431          
## 561 610/20-A 1427          
## 561 780/60-A 1432          
## 405 585/15-A  766 1638

Channels excluded from the analysis: FSC-A, FSC-W, SSC-A, SSC-W.

No changepoints were detected in the channels: 355 379/28-A, 355 740/35-A, 355 820/60-A.

Dynamic range check

1.12 % anomalies detected in dynamic range check.

The plot shows where the anomalies occured the most. The x-axis scale is complementary to the one of the signal acquisition plot.

More info on the anomalies detected in the dynamic range check

For each channel analyzed, the table shows the number of events that did not pass the dynamic range check:

##              lower_range upper_range
## 488 525/50-A         114        2834
## 488 715/30-A          32          86
## 405 450/50-A          71         450
## 405 515/20-A          52        2929
## 405 605/40-A          40         146
## 405 670/30-A          47          83
## 405 710/40-A          51          63
## 405 780/60-A         262           6
## 355 379/28-A          70         373
## 355 515/30-A          69         841
## 355 560/40-A          75         277
## 355 740/35-A          25          10
## 355 820/60-A         359           3
## 640 670/30-A          31          31
## 640 730/35-A          47          18
## 640 780/60-A          60          39
## 561 585/15-A          53        8019
## 561 610/20-A          34        3742
## 561 780/60-A          41          30
## 405 585/15-A          50         262
## total_SUM           1583       20242
## total_UNIQUE         665       10911